Medium data
Search for medium no. = [1406].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1406 TEPIDIBACTER 8C15B MEDIUM
| NaCl | 18.0 | g |
| MgCl2·6H2O | 4.0 | g |
| KCl | 0.34 | g |
| NH4Cl | 0.25 | g |
| CaCl2·2H2O | 0.11 | g |
| K2HPO4 | 0.18 | g |
| FeCl2 solution (see Medium No. 187) | 1.0 | ml |
| Trace element solution (see Medium No. 187) | 1.0 | ml |
| Selenite-tungstate solution (see Medium No. 431) | 1.0 | ml |
| Yeast extract | 0.2 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 950.0 | ml |
Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, aseptically and anaerobically, add per liter the following solutions (autoclaved or *filter-sterilized):
| 8% NaHCO3 solution* | 30.0 | ml |
| 1 M Glucose solution | 20.0 | ml |
Dispense the medium into culture vessels (e.g. 20 ml in 50 ml serum bottles) under the same gas mixture and seal with butyl rubber stoppers. Finally, add per liter the following solution (autoclaved and stored under a N2 atmosphere):
| 5% Na2S·9H2O solution | 10.0 | ml |
Adjust pH to 7.0, if necessary.
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