8% NaHCO3 solution* | 30.0 | ml |
1 M MgCl2·6H2O solution | 17.0 | ml |
1 M CaCl2·2H2O solution | 1.0 | ml |
Vitamin solution (see Medium No. 403)* | 1.0 | ml |
Thiamine solution (see Medium No. 403)* | 1.0 | ml |
Vitamin B12 solution (see Medium No. 403)* | 1.0 | ml |
1 M Sodium formate solution | 5.0 | ml |
1 M Sodium acetate solution | 2.0 | ml |
Aseptically and anaerobically, distribute the medium into culture vessels under the same gas mixture (e.g., 20 ml in 60 ml-serum bottles) and seal with butyl rubber stoppers. Prior to use, add per liter the following solution to reduce the medium (autoclaved and stored under N