Medium data
Search for medium no. = [1396].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1396 METHANOCOCCOIDES MEDIUM 2
| KCl | 0.34 | g |
| MgCl2·6H2O | 4.0 | g |
| NH4Cl | 0.25 | g |
| CaCl2·2H2O | 0.14 | g |
| KH2PO4 | 0.14 | g |
| NaCl | 9.0 | g |
| Wolfe's mineral solution (see Medium No. 265) | 10.0 | ml |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| Fe(NH4)2(SO4)2·6H2O | 2.0 | mg |
| NaHCO3 | 5.0 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Mix components thoroughly, except NaHCO3, and adjust pH to 6.5. Bring to a boil for 5 -10 sec, then, cool down under a stream of N2-CO2 (80:20, v/v) and add NaHCO3 to the medium. Dispense the medium into suitable culture vessels (e.g., 5 ml medium in Hungate tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Stand the medium overnight. Aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized, and stored under a N2 atmosphere):
| 3% Trimethylamine solution* | 10.0 | ml |
| 50% (v/v) Methanol solution* | 10.0 | ml |
| 5% L-Cysteine·HCl·H2O solution | 10.0 | ml |
| 5% Na2S·9H2O solution | 10.0 | ml |
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