Medium data

Search for medium no. = [1387].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


KH2PO4  0.80     g
CaCl2·2H2O  0.05     g
NH4Cl  0.80     g
MgCl2·6H2O  0.10     g
NaCl  120.00     g
Na2SO4  15.00     g
NaHCO3  10.00     g
Na2CO3  5.00     g
Trace element solution SLA (see Medium No. 358)  1.00     ml
Na2S·9H2O  0.50     g
Sodium acetate  1.00     g
Distilled water  1000.00     ml
Adjust pH to 8.5
Bubble with nitrogen gas and fill in tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121C for 15 min. After sterilization add 1 ml/l filter-sterilized vitamin solution VA (see Medium No. 358) and appropriate amount of a sterilized Na2S·9H2O solution. Adjust pH to 8.5.
Na2S·9H2O solution:
Na2S·9H2O  10.0      g
Distilled water  100.0      ml

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