Medium data

Search for medium no. = [1387].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1387 ECTOTHIORHODOSPIRA ABDELMALEKII MEDIUM

KH₂PO₄	`0.80`	g
CaCl₂·2H₂O	`0.05`	g
NH₄Cl	`0.80`	g
MgCl₂·6H₂O	`0.10`	g
NaCl	`120.00`	g
Na₂SO₄	`15.00`	g
NaHCO₃	`10.00`	g
Na₂CO₃	`5.00`	g
Trace element solution SLA (see Medium No. 358)	`1.00`	ml
Na₂S·9H₂O	`0.50`	g
Sodium acetate	`1.00`	g
Distilled water	`1000.00`	ml

Adjust pH to 8.5
Bubble with nitrogen gas and fill in tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121C for 15 min. After sterilization add 1 ml/l filter-sterilized vitamin solution VA (see Medium No. 358) and appropriate amount of a sterilized Na₂S·9H₂O solution. Adjust pH to 8.5.
Na₂S·9H₂O solution:

Na₂S·9H₂O	`10.0`	g
Distilled water	`100.0`	ml