Medium data
Search for medium no. = [358].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
358 ATYP MEDIUM
| Trace element solution SLA (see below) | 1.0 | ml |
| Vitamin solution VA (see below) | 1.0 | ml |
| KH2PO4 | 1.0 | g |
| MgCl2·6H2O | 0.5 | g |
| CaCl2·2H2O | 0.1 | g |
| NH4Cl | 1.0 | g |
| NaCl | 1.0 | g |
| Sodium acetate | 1.0 | g |
| Sodium succinate | 1.0 | g |
| Yeast extract (BD-Difco) | 0.5 | g |
| Bacto peptone (BD-Difco) | 0.3 | g |
| Distilled water | 1.0 | L |
Adjust pH to 6.9.
Separately sterilize vitamin solution VA by filtration, and aseptically add to the medium.
Trace element solution SLA:
| FeCl2·4H2O | 1800.0 | mg |
| CoCl2·6H2O | 250.0 | mg |
| NiCl2·6H2O | 10.0 | mg |
| CuCl2·2H2O | 10.0 | mg |
| MnCl2·4H2O | 70.0 | mg |
| ZnCl2 | 100.0 | mg |
| H3BO3 | 500.0 | mg |
| Na2MoO4·2H2O | 30.0 | mg |
| Na2SeO3·5H2O | 10.0 | mg |
| Distilled water | 1.0 | L |
Adjust pH to 2 - 3 with HCl.
Vitamin solution VA:
| Biotin | 10.0 | mg |
| Niacin | 35.0 | mg |
| Thiamine·HCl | 30.0 | mg |
| p-Aminobenzoic acid | 20.0 | mg |
| Pyridoxine·HCl | 10.0 | mg |
| Calcium pantothenate | 10.0 | mg |
| Vitamin B12 | 5.0 | mg |
| Distilled water | 100.0 | ml |
Copyright © 2023 Microbe Division (JCM) - All Rights Reserved