Medium data

Search for medium no. = [358].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

358   ATYP MEDIUM

Trace element solution SLA (see below)  1.0     ml
Vitamin solution VA (see below)  1.0     ml
KH2PO4  1.0     g
MgCl2·6H2O  0.5     g
CaCl2·2H2O  0.1     g
NH4Cl  1.0     g
NaCl  1.0     g
Sodium acetate  1.0     g
Sodium succinate  1.0     g
Yeast extract (BD-Difco)  0.5     g
Bacto peptone (BD-Difco)  0.3     g
Distilled water  1.0     L
Adjust pH to 6.9.
Separately sterilize vitamin solution VA by filtration, and aseptically add to the medium.
Trace element solution SLA:
FeCl2·4H2O  1800.0     mg
CoCl2·6H2O  250.0     mg
NiCl2·6H2O  10.0     mg
CuCl2·2H2O  10.0     mg
MnCl2·4H2O  70.0     mg
ZnCl2  100.0     mg
H3BO3  500.0     mg
Na2MoO4·2H2O  30.0     mg
Na2SeO3·5H2O  10.0     mg
Distilled water  1.0     L
Adjust pH to 2 - 3 with HCl.
Vitamin solution VA:
Biotin  10.0     mg
Niacin  35.0     mg
Thiamine·HCl  30.0     mg
p-Aminobenzoic acid  20.0     mg
Pyridoxine·HCl  10.0     mg
Calcium pantothenate  10.0     mg
Vitamin B12  5.0     mg
Distilled water  100.0     ml