Medium data

Search for medium no. = [1386].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1386 ECTOTHIORHODOSPIRA MEDIUM

KH₂PO₄	`0.50`	g
CaCl₂·2H₂O	`0.05`	g
NH₄Cl	`0.80`	g
MgCl₂·6H₂O	`0.10`	g
NaCl	`180.00`	g
Na₂SO₄	`20.00`	g
Na₂CO₃	`6.00`	g
Trace element solution SLA (see Medium No. 358)	`1.00`	ml
Yeast extract (BD-Difco)	`0.50`	g
Sodium succinate	`1.00`	g
Distilled water	`800.00`	ml

Adjust pH to 8.5.
Bubble with nitrogen gas and fill in tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121C for 15 min. After sterilization add 1 ml/l filter-sterilized vitamin solution VA (see Medium No. 358), 200 ml of filter-sterilized Bicarbonate solution and 10 ml/l sterilized Na₂S·9H₂O solution. Adjust pH to 8.5.
Bicarbonate solution:

NaHCO₃	`14.0`	g
Distilled water	`200.0`	ml

Na₂S·9H₂O solution:

Na₂S·9H₂O	`10.0`	g
Distilled water	`100.0`	ml