Medium data

Search for medium no. = [1380].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1380 EUGON AGAR WITH 5% SHEEP BLOOD AND ARTIFICIAL SEAWATER

Eugon agar (BD-Difco)	`45.4`	g
Sheep blood	`50.0`	ml
Artificial seawater (see Medium No. 736)	`950.0`	ml

Mix ingredients except sheep blood, autoclave and cool to about 45C. Aseptically add 5% (final) sterile defibrinated sheep blood. Mix and quickly dispense into sterile petri dishes.