Medium data
Search for medium no. = [736].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
736 1/2 R2A MEDIUM WITH 75% ARTIFICIAL SEAWATER
Yeast extract (BD-Difco) | 0.25 | g |
Proteose peptone No. 3 (BD-Difco) | 0.25 | g |
Casamino acids (BD-Difco) | 0.25 | g |
Glucose | 0.25 | g |
Soluble starch | 0.25 | g |
Sodium pyruvate | 0.15 | g |
K2HPO4 | 0.15 | g |
MgSO4·7H2O | 0.025 | g |
Distilled water | 250.0 | ml |
Artificial seawater (see below) | 750.0 | ml |
Mix components except artificial seawater, adjust pH to 7.2, and autoclave. Separately autoclave artificial seawater. After cooling, combine the two solutions.
Artificial seawater:
NaCl | 23.5 | g |
MgCl2·6H2O | 4.9 | g |
Na2SO4 | 3.9 | g |
CaCl2·2H2O | 1.1 | g |
KCl | 0.66 | g |
NaHCO3 | 0.19 | g |
KBr | 0.096 | g |
H3BO3 | 0.026 | g |
SrCl2·6H2O | 0.024 | g |
NaF | 0.003 | g |
Distilled water | 1.0 | L |
Copyright © 2025 Microbe Division (JCM) - All Rights Reserved