Medium data

Search for medium no. = [736].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

736   1/2 R2A MEDIUM WITH 75% ARTIFICIAL SEAWATER

Yeast extract (BD-Difco)  0.25    g
Proteose peptone No. 3 (BD-Difco)  0.25    g
Casamino acids (BD-Difco)  0.25    g
Glucose  0.25    g
Soluble starch  0.25    g
Sodium pyruvate  0.15    g
K2HPO4  0.15    g
MgSO4·7H2O  0.025   g
Distilled water  250.0     ml
Artificial seawater (see below)  750.0     ml
Adjust pH to 7.2.
Artificial seawater:
NaCl  23.5     g
MgCl2·6H2O  4.9     g
Na2SO4  3.9     g
CaCl2·2H2O  1.1     g
KCl  0.66    g
NaHCO3  0.19    g
KBr  0.096   g
H3BO3  0.026   g
SrCl2·6H2O  0.024   g
NaF  0.003   g
Distilled water  1.0     L