Medium data

Search for medium no. = [1366].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1366 GBS SALTS MEDIUM FOR HYDROGENOBACTER

NaCl	`3.0`	g
KCl	`0.15`	g
Na₂SO₄	`0.3`	g
MgSO₄·7H₂O	`0.123`	g
CaCl₂·2H₂O	`14.5`	mg
NH₄Cl	`0.107`	g
Mineral solution A (see Medium No. 1353)	`5.0`	ml
Distilled water	`1.0`	L

Mix components thoroughly and distribute the medium into culture vessels (e.g., 5.0 ml in 25 ml serum bottles/Balch tubes) under a stream of N₂, seal with butyl rubber stoppers and autoclave. After cooling, aseptically add per liter the following solutions (autoclaved or *filter-sterilized and stored anaerobically):

8% NaHCO₃ solution*	`12.5`	ml
1.0 M Na₂S₂O₃ solution	`2.5`	ml
0.5 M Na₂HPO₄ solution (pH 8.0)	`20.0`	ml

After inoculation, add air to the gas phase (30-40% by volume) and pressurize to 100 KPa with H₂-CO₂ (4:1, v/v) gas mixture.