Medium data
Search for medium no. = [1353].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1353 GBS SALTS MEDIUM FOR FERVIDIBACTER
| NaCl | 3.0 | g |
| KCl | 0.15 | g |
| Na2SO4 | 0.3 | g |
| MgSO4·7H2O | 0.123 | g |
| CaCl2·2H2O | 14.5 | mg |
| NH4Cl | 21.4 | mg |
| HEPES | 0.477 | g |
| Mineral solution A (see below) | 5.0 | ml |
| Distilled water | 1.0 | L |
Mix components thoroughly and adjust pH to 7.5 with NaOH. Distribute the medium into culture vessels (e.g., 5.0 ml in 25 ml serum bottles/Balch tubes) under a stream of N2, seal with butyl rubber stoppers and autoclave. After cooling, aseptically add per liter the following solutions (autoclaved or filter-sterilized):
| Trace vitamins solution* (see below) | 10.0 | ml |
| 4% Glycogen solution | 10.0 | ml |
| Mineral solution B* (see below) | 2.0 | ml |
Prior to inoculation, add filter-sterilized O2 gas or air, to make a final concentration of 10% O2 in the gas phase.
Mineral solution A:
| EDTA | 2.5 | g |
| KOH | 2.2 | g |
| FeSO4·7H2O | 1.1 | g |
| MnCl2·4H2O | 98.4 | mg |
| ZnSO4·7H2O | 28.8 | mg |
| CoCl2·6H2O | 23.8 | mg |
| CuCl2·2H2O | 17.2 | mg |
| Na2MoO4·2H2O | 24.2 | mg |
| H3BO3 | 31.0 | mg |
| Distilled water | 1.0 | L |
Adjust pH to 6.5 with HCl.
Trace vitamins solution:
| Trace vitamins (see Medium No. 197) | 1.0 | L |
| NaH2PO4 | 0.468 | g |
| Na2HPO4 | 0.866 | g |
Mineral solution B:
| Na2WO4·2H2O | 1.65 | g |
| Ni(NH4)2(SO4)2·6H2O | 19.8 | mg |
| VOSO4 | 0.82 | mg |
| Distilled water | 1.0 | L |
Copyright © 2025 Microbe Division (JCM) - All Rights Reserved