Medium data

Search for medium no. = [1354].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1354 RFODOSPIRILLACEAE MEDIUM (MODIFIED)

Yeast extract (BD-Difco)	`0.3`	g
Sodium succinate	`1.0`	g
Ammonium acetate	`0.5`	g
0.1% Fe(III) citrate solution	`5.0`	ml
KH₂PO₄	`0.5`	g
MgSO₄·7H₂O	`0.4`	g
NaCl	`0.4`	g
NH₄Cl	`0.4`	g
CaCl₂·2H₂O	`0.05`	g
0.01% Vitamin B₁₂ solution	`0.4`	ml
SL-6 Trace element solution (see Medium No. 167)	`1.0`	ml
L-Cysteine·HCl·H₂O	`0.3`	g
Resazurin	`0.5`	mg
Distilled water	`1.0`	L

Mix components thoroughly and adjust pH 6.8. Bring to a boil, cool down under a stream of N₂, distribute the medium into culture vessels (e.g., 10 ml medium in Hungate type tubes) under N₂, seal with butyl rubber stoppers and autoclave.
After inoculation, cultivate in the light using a tungsten lamp.