Medium data
Search for medium no. = [1340].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1340 MODIFIED PFENNIG MEDIUM WITH GLUCOSE
| KH2PO4 | 0.16 | g |
| NH4Cl | 0.16 | g |
| MgCl2·6H2O | 0.16 | g |
| KCl | 0.16 | g |
| CaCl2·2H2O | 0.1 | g |
| MOPS | 2.0 | g |
| Glucose | 2.0 | g |
| Yeast extract | 0.1 | g |
| Distilled water | 1.0 | L |
Mix components thoroughly, adjust pH to 6.7-7.0 and autoclave. After cooling, aseptically add the following solutions (autoclaved or *filter-sterilized):
| Vitamin solution* (see Medium No. 899) | 1.0 | ml |
| Trace element solution* (see Medium No. 899) | 1.0 | ml |
Cultivate without shaking.
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