Medium data

Search for medium no. = [1337].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1337 MODIFIED BN MEDIUM FOR M08DHB

NH₄Cl	`0.33`	g
KCl	`0.33`	g
KH₂PO₄	`0.33`	g
MgCl₂·6H₂O	`0.33`	g
CaCl₂·2H₂O	`0.033`	g
NaCl	`5.0`	g
Trace element solution (see Medium No. 1030)	`1.0`	ml
Resazurin	`1.0`	mg
Distilled water	`940.0`	ml

Mix components thoroughly and autoclave under a N₂-CO₂ (80:20, v/v) gas mixture. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):

8% NaHCO₃ solution*	`25.0`	ml
1.0 M Sodium formate solution	`10.0`	ml
1.0 M Sodium thiosulfate solution	`10.0`	ml
1.0% yeast extract solution	`5.0`	ml
Trace vitamins* (see Medium No. 197)	`1.0`	ml
5% Na₂S·9H₂O solution	`10.0`	ml

Adjust pH to 7.5, if necessary.