Medium data

Search for medium no. = [1330].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1330   BY MEDIUM

NaCl  1.0     g
K2HPO4  0.5     g
(NH4)2SO4  0.25    g
CaCl2·2H2O  0.13    g
MgSO4·7H2O  0.2     g
KH2PO4  1.0     g
Rumen fluid, clarified (see below)  300.0     ml
Cellobiose  5.0     g
Yeast extract  1.0     g
NaHCO3  5.0     g
L-Cysteine·HCl·H2O  0.5     g
Trace element solution SL-10 (see Medium No. 433)  1.0     ml
Resazurin  1.0     mg
Distilled water  700.0     ml
Adjust pH to 7.0
Mix ingredients except NaHCO3 and L-Cysteine·HCl·H2O. Bring to a boil and cool under CO2 gas stream, then add NaHCO3 and L-Cysteine·HCl·H2O to the medium. Dispense the medium into suitable culture vessels under a stream of CO2, seal with butyl rubber stoppers, and autoclave.
To prepare the clarified rumen fluid, preheat rumen content at 120C for 15 min. Use the supernatant after centrifuging at 25,000 x g for 15 min.

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