Medium data

Search for medium no. = [1330].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1330 BY MEDIUM

NaCl	`1.0`	g
K₂HPO₄	`0.5`	g
(NH₄)₂SO₄	`0.25`	g
CaCl₂·2H₂O	`0.13`	g
MgSO₄·7H₂O	`0.2`	g
KH₂PO₄	`1.0`	g
Rumen fluid, clarified (see below)	`300.0`	ml
Cellobiose	`5.0`	g
Yeast extract	`1.0`	g
NaHCO₃	`5.0`	g
L-Cysteine·HCl·H₂O	`0.5`	g
Trace element solution SL-10 (see Medium No. 433)	`1.0`	ml
Resazurin	`1.0`	mg
Distilled water	`700.0`	ml

Adjust pH to 7.0
Mix ingredients except NaHCO₃ and L-Cysteine·HCl·H₂O. Bring to a boil and cool under CO₂ gas stream, then add NaHCO₃ and L-Cysteine·HCl·H₂O to the medium. Dispense the medium into suitable culture vessels under a stream of CO₂, seal with butyl rubber stoppers, and autoclave.
To prepare the clarified rumen fluid, preheat rumen content at 120C for 15 min. Use the supernatant after centrifuging at 25,000 x g for 15 min.