Medium data
Search for medium no. = [1328].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1328 PROLIXIBACTER MEDIUM
| NaCl | 18.0 | g |
| MgCl2·6H2O | 2.6 | g |
| CaCl2·2H2O | 0.15 | g |
| NH4Cl | 0.25 | g |
| KH2PO4 | 0.2 | g |
| KCl | 0.5 | g |
| NaNO3 | 0.85 | g |
| Glucose | 1.8 | g |
| Yeast extract (BD-Difco) | 1.0 | g |
| Trace minerals (see Medium No. 243) | 1.0 | ml |
| Trace vitamins (see Medium No. 197) | 1.0 | ml |
| NaHCO3 | 2.52 | g |
| Distilled water | 1.0 | L |
Mix components, and adjust pH to 7.0. Dispense the medium into suitable culture vessels under a stream of N2-CO2 (80:20, v/v), seal with butyl rubber stoppers, and autoclave. Prior to inoculation, aseptically and anaerobically add per liter the following solutions (autoclaved and stored under a N2 atmosphere):
| 5% Na2S·9H2O | 10.0 | ml |
| 5% L-Cysteine·HCl·H2O | 10.0 | ml |
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