Medium data

Search for medium no. = [1307].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1307 PPLL MEDIUM

NaCl	`1.0`	g
MgCl₂·6H₂O	`0.4`	g
CaCl₂·2H₂O	`0.1`	g
Na₂SO₄	`4.0`	g
NH₄Cl	`0.25`	g
KH₂PO₄	`0.2`	g
KCl	`0.5`	g
Yeast extract	`0.5`	g
Sodium malate	`0.89`	g
Distilled water	`1.0`	L

Mix components and autoclave. Cool down under a N₂ gas stream. Aseptically and anaerobically add per liter the following solutions from sterile anaerobic stocks :

Trace element solution (see Medium No. 439)	`1.0`	ml
Selenite-tungstate solution (see Medium No. 431)	`1.0`	ml
8% NaHCO₃ solution	`30.0`	ml
Vitamin solution (see Medium No. 403)	`1.0`	ml
Thiamine solution (see Medium No. 403)	`1.0`	ml
Vitamin B₁₂ solution (see Medium No. 403)	`1.0`	ml

Adjust pH to 7.0-7.3. Aseptically and anaerobically distribute the medium into culture vessels (e.g., 10 ml medium in Balch tubes), fill the head space with a gas mixture of N₂-CO₂ (4:1, v/v), and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution.

5% Na₂S·9H₂O solution	`5.0`	ml
5% L-Cysteine·HCl·H₂O solution	`5.0`	ml