Medium data

Search for medium no. = [1298].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1298   ABSS2

NaCl  11.8     g
MgCl2·6H2O  2.2     g
CaCl2·2H2O  0.3     g
KCl  0.4     g
Distilled water  1.0     L
Mix components and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized):
1 M NaHCO3 solution*  3.0     ml
100 mM NH4Cl solution  20.0     µl
10 mM Na2HPO4 solution  100.0     µl
SL-7 trace element solution (see Medium No. 536)  100.0     µl
Vitamin solution* (see below)  420.0     µl
300 mM Na2S2O3 solution  10.0     ml
500 mM MnO2 solution  10.0     ml
Adjust pH to 7.5 by bubbling with a N2-CO2 (4:1, v/v) gas mixture.
Vitamin solution:
Vitamin B12  10.0     mg
Inositol  10.0     mg
Biotin  10.0     mg
Folic acid  10.0     mg
p-Aminobenzoic acid  100.0     mg
Nicotinic acid  1.0     g
DL-Calcium pantothenate  1.0     g
Thiamine-HCl  2.0     g
Distilled water  1.0     L