Medium data
Search for medium no. = [1293].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1293 MMJF MEDIUM
| Modified MJ synthetic seawater C (see below) | 1.0 | L |
| Ferrihydrite | 2.0 | g |
Mix components and adjust pH to 6.5. Distribute the medium into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solution (filter-sterilized):
| 8% NaHCO3 solution | 12.5 | ml |
Check final pH of the medium to be 6.5.
Modified MJ synthetic seawater C:
| NaCl | 25.0 | g |
| MgCl2·6H2O | 4.2 | g |
| MgSO4·7H2O | 3.4 | g |
| KCl | 0.5 | g |
| NH4Cl | 0.25 | g |
| K2HPO4 | 0.14 | g |
| CaCl2·2H2O | 0.7 | g |
| FeSO4·7H2O | 0.02 | g |
| Trace mineral solution (see below) | 10.0 | ml |
| Distilled water | 1.0 | L |
Trace mineral solution:
| Trace minerals (see Medium No. 151) | 1.0 | L |
| NiCl2·6H2O | 0.075 | g |
| Na2SeO3·5H2O | 0.05 | g |
| Na2WO4·2H2O | 0.1 | g |
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