Medium data
Search for medium no. = [1282].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1282 HNN MEDIUM
| NaCl | 25.0 | g |
| MgCl2·6H2O | 4.2 | g |
| MgSO4·7H2O | 3.4 | g |
| KCl | 0.5 | g |
| NH4Cl | 0.25 | g |
| K2HPO4 | 0.14 | g |
| CaCl2·2H2O | 0.7 | g |
| NaHCO3 | 1.0 | g |
| Trace mineral solution (see below) | 10.0 | ml |
| Distilled water | 1.0 | L |
Mix components, except NaHCO3, and autoclave under N2 atmosphere. Filter-sterilize 8% (w/v) NaHCO3 solution and add to the medium. Aseptically distribute the medium into culture vessels and seal with butyl rubber stoppers. Replace gas phase with N2O, After inoculation, pressurize the inoculated bottles to 300 kPa H2-CO2 (4:1, v/v).
Trace mineral solution:
| Trace minerals (see Medium No. 151) | 1.0 | L |
| NiCl2·6H2O | 0.025 | g |
| Na2SeO3·5H2O | 0.3 | mg |
| Na2WO4·2H2O | 0.1 | g |
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