Medium data

Search for medium no. = [1273].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1273   0.01% LB SEAWATER MEDIUM

NaNO3  120.0     mg
K2HPO4  5.0     mg
Vitamin B12  1.0     μg
Biotin  1.0     μg
Thiamine·HCl  100.0     μg
Fe(III)·EDTA  259.0     μg
Mn(II)·EDTA  332.0     μg
Tris(hydroxymethyl)aminomethane  1.0     g
LB Broth, Lennox (BD-Difco)  0.1     g
Seawater  1.0     L
Mix components thoroughly, adjust pH to 8.0 with HCl and autoclave.
Comment: Artificial sea water (such as artificial sea water in Medium No. 1118) can be used instead of natural sea water.