Medium data
Search for medium no. = [1273].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1273 0.01% LB SEAWATER MEDIUM
| NaNO3 | 120.0 | mg |
| K2HPO4 | 5.0 | mg |
| Vitamin B12 | 1.0 | μg |
| Biotin | 1.0 | μg |
| Thiamine·HCl | 100.0 | μg |
| Fe(III)·EDTA | 259.0 | μg |
| Mn(II)·EDTA | 332.0 | μg |
| Tris(hydroxymethyl)aminomethane | 1.0 | g |
| LB Broth, Lennox (BD-Difco) | 0.1 | g |
| Seawater | 1.0 | L |
Mix components thoroughly, adjust pH to 8.0 with HCl and autoclave.
Comment: Artificial sea water (such as artificial sea water in Medium No. 1118) can be used instead of natural sea water.
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