Medium data

Search for medium no. = [1118].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1118 SULFURIMONAS JS MEDIUM

NH₄NO₃	`0.1`	g
KH₂PO₄	`0.01`	g
Fe(III)·EDTA	`2.5`	mg
Vitamin mixture(see below)	`2.5`	ml
Metal mixture (see below)	`1.0`	ml
PIPES	`15.05`	g
Artificial seawater (see below)	`1.0`	L

Mix components, adjust pH to 6.5 with NaOH, then distribute the medium into culture vessels (e.g., 50 ml per 120 ml serum bottles), seal with butyl rubber stoppers and autoclave at 110C for 5 min. After cooling, add per liter the following solutions (filter-sterilized):

8% NaHCO₃ solution	`10.0`	ml
1 M Na₂S₂O₃ solution	`10.0`	ml

Vitamin mixture:

Vitamin B₁₂	`1.1`	mg
Biotin	`1.0`	mg
Thiamine·HCl	`200.0`	mg
Distilled water	`1.0`	L

Metal mixture:

EDTA·2Na	`0.372`	g
CuSO₄·5H₂O	`0.25`	mg
ZnSO₄·7H₂O	`5.75`	mg
MnCl₂·6H₂O	`4.55`	mg
CoCl₂·6H₂O	`0.6`	mg
(NH₄)Mo₇O₂₄·4H₂O	`0.27`	mg
Distilled water	`1.0`	L

Artificial seawater:

NaCl	`26.3`	g
CaCl₂·2H₂O	`1.54`	g
KBr	`0.1`	g
KF	`0.004`	g
KCl	`0.7`	g
H₃BO₃	`30.0`	mg
MgSO₄·7H₂O	`7.0`	g
KHCO₃	`0.24`	g
SrCl₂·6H₂O	`17.0`	mg
MgCl₂·6H₂O	`5.2`	g
Distilled water	`1.0`	L

Artificial seawater can be replaced with natural seawater. In this case, filtrate seawater (pore size, 02 µm) and boil. After cooling, filtrate again (pore size, 0.45 µm).