Medium data

Search for medium no. = [1267].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1267 MODIFIED PICROPHILUS MEDIUM

Mix components thoroughly, adjust pH to 2.0 - 2.5 with H₂SO₄ and autoclave. After cooling, add the following solutions (filter-sterilized):

10% Tryptone solution	`11.1`	ml
10% Yeast extract solution	`11.1`	ml
10% Fe₂(SO₄)₃ solution (in 0.01 N H₂SO₄ solution)	`88.8`	ml
Wolfe's mineral elixir (see Medium No. 470)	`2.0`	ml
Vitamin solution (see below)	`2.0`	ml

Vitamin solution:

Stir for several hours to dissolve.