Medium data

Search for medium no. = [1257].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1257 DESULFOBACTERIUM CATECHOLICUM MEDIUM

Na₂SO₄	`3.0`	g
KH₂PO₄	`0.2`	g
NH₄Cl	`0.3`	g
NaCl	`7.0`	g
MgCl₂·6H₂O	`1.3`	g
KCl	`0.5`	g
CaCl₂·2H₂O	`0.15`	g
FeCl₂ solution (see Medium No. 187)	`1.0`	ml
Trace element solution (see Medium No. 187)	`1.0`	ml
Selenite-tungstate solution (see Medium No. 431)	`1.0`	ml
Resazurin	`0.5`	mg
Distilled water	`1.0`	L

Mix components thoroughly, bring to a boil and cool down under a N₂-CO₂ (4:1, v/v) gas mixture. Distribute the medium into culture vessels (e.g., 10 ml in Balch tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized and stored under a N₂ gas atmosphere; **filter-sterilized and stored under an 80% N₂-20% CO₂ gas mixture):

Trace vitamins* (see Medium No. 197)	`10.0`	ml
4% Sodium benzoate solution*	`10.0`	ml
Pyrocatechol solution* (see below)	`10.0`	ml
8% NaHCO₃ solution**	`20.0`	ml
5% Na₂S·9H₂O solution	`8.0`	ml

Pyrocatechol solution:

Pyrocatechol	`0.06`	g
1 N HCl	`0.06`	ml
Distilled water	`10.0`	ml

Filter-sterilize. Use always freshly prepared solution prior to inoculation.