Medium data

Search for medium no. = [1238].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1238   TANNER MOD2 MEDIUM

NaCl  2.0     g
NH4Cl  0.25    g
KCl  0.25    g
KH2PO4  0.25    g
MgSO4·7H2O  0.5     g
CaCl2·2H2O  0.1     g
MOPS  2.09    g
Yeast extract  0.5     g
SL-9 mineral solution (see below)  10.0     ml
L-Cysteine·HCl·H2O  0.5     g
Resazurin  0.5     mg
Distilled water  990.0     ml
Mix components thoroughly and adjust pH 7.0. Dispense the medium into culture vessels under a N2 gas atmosphere, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from sterile anaerobic stocks (filter-sterilized):
1 M Fructose solution  30.0     ml
Trace vitamins(see Medium No. 197)  10.0     ml
Vitamin B12 solution (see Medium No. 403)  1.0     ml
0.01% Coenzyme M solution  1.0     ml
SL-9 mineral solution:
Nitrilotriacetic acid  2.0     g
MnSO4·xH2O  1.0     g
Fe(NH4)2(SO4)2·6H2O  0.8     g
CoCl2·6H2O  0.2     g
ZnSO4·7H2O  1.0     mg
CuCl2·2H2O  20.0     mg
NiCl2·6H2O  20.0     mg
Na2MoO4·2H2O  20.0     mg
Na2SeO4  20.0     mg
Na2WO4·2H2O  20.0     mg
Distilled water  1.0     L
Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0.



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