Medium data

Search for medium no. = [1238].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1238 TANNER MOD2 MEDIUM

NaCl	`2.0`	g
NH₄Cl	`0.25`	g
KCl	`0.25`	g
KH₂PO₄	`0.25`	g
MgSO₄·7H₂O	`0.5`	g
CaCl₂·2H₂O	`0.1`	g
MOPS	`2.09`	g
Yeast extract	`0.5`	g
SL-9 mineral solution (see below)	`10.0`	ml
L-Cysteine·HCl·H₂O	`0.5`	g
Resazurin	`0.5`	mg
Distilled water	`990.0`	ml

Mix components thoroughly and adjust pH 7.0. Dispense the medium into culture vessels under a N₂ gas atmosphere, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from sterile anaerobic stocks (filter-sterilized):

1 M Fructose solution	`30.0`	ml
Trace vitamins(see Medium No. 197)	`10.0`	ml
Vitamin B₁₂ solution (see Medium No. 403)	`1.0`	ml
0.01% Coenzyme M solution	`1.0`	ml

SL-9 mineral solution:

Nitrilotriacetic acid	`2.0`	g
MnSO₄·xH₂O	`1.0`	g
Fe(NH₄)₂(SO₄)₂·6H₂O	`0.8`	g
CoCl₂·6H₂O	`0.2`	g
ZnSO₄·7H₂O	`1.0`	mg
CuCl₂·2H₂O	`20.0`	mg
NiCl₂·6H₂O	`20.0`	mg
Na₂MoO₄·2H₂O	`20.0`	mg
Na₂SeO₄	`20.0`	mg
Na₂WO₄·2H₂O	`20.0`	mg
Distilled water	`1.0`	L

Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0.