Medium data

Search for medium no. = [1219].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1219 METHYLOTROPH MEDIUM

KH₂PO₄	`0.27`	g
NaNO₃	`0.42`	g
MgCl₂·6H₂O	`0.20`	g
CaCl₂·2H₂O	`0.07`	g
Na₂SO₄	`0.014`	g
HEPES	`4.77`	g
Trace element solution (see Medium No. 284)	`10.0`	ml
Se/W solution (see Medium No. 284)	`10.0`	ml
Trace vitamins (see Medium No. 197)	`10.0`	ml
Distilled water	`970.0`	ml

Mix components and adjust pH to 7.0 with 6 N KOH. For preparation of solid medium, add 15.0 g agar. Autoclave. After cooling, add the following solutions (filer-sterilized):

Methanol	`0.81`	ml
10 mM CuCl₂ solution	`2.0`	ml
10 mM CeCl₃ solution	`2.0`	ml