Medium data

Search for medium no. = [1219].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1219   METHYLOTROPH MEDIUM

KH2PO4  0.27    g
NaNO3  0.42    g
MgCl2·6H2O  0.20    g
CaCl2·2H2O  0.07    g
Na2SO4  0.014   g
HEPES  4.77    g
Trace element solution (see Medium No. 284)  10.0     ml
Se/W solution (see Medium No. 284)  10.0     ml
Trace vitamins (see Medium No. 197)  10.0     ml
Distilled water  970.0     ml
Mix components and adjust pH to 7.0 with 6 N KOH. For preparation of solid medium, add 15.0 g agar. Autoclave. After cooling, add the following solutions (filer-sterilized):
Methanol  0.81    ml
10 mM CuCl2 solution  2.0     ml
10 mM CeCl3 solution  2.0     ml