Medium data

Search for medium no. = [1200].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1200   ELBE METHANOTROPH MEDIUM

MgCl2·6H2O  0.93    g
KCl  1.0     g
CaCl2·2H2O  0.12    g
NaNO3  0.17    g
Distilled water  992.0     ml
Mix components and autoclave. For preparation of solid medium, add 15.0 g/L agar before autoclaving. After cooling to 55C, add the following solutions (autoclaved or *filter-sterilized) to the medium:
2 M HEPES buffer (pH 7.0)  5.0     ml
1 M Phosphate buffer (see Medium No. 556)  0.15    ml
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Vitamin solution* (see Medium No. 556)  1.0     ml
Distribute the culture medium into culture tubes (leave > 80% volume of a gas phase)and seal with butyl rubber stoppers. Finally, add methane to make a concentration of 50% in the gas phase (by volume).



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