Medium data
Search for medium no. = [1197].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1197 ACIDIHALOBACTER PROSPERUS F5 MEDIUM
Solution A:
NaCl | 25.0 | g |
(NH4)2SO4 | 0.4 | g |
MgSO4·7H2O | 0.4 | g |
K2HPO4 | 0.4 | g |
Distilled water | 900.0 | ml |
Adjust pH 2.5 with H2SO4 and autoclave.
Solution B:
FeSO4·7H2O | 13.9 | g |
K2S4O6 | 1.51 | g |
Distilled water | 100.0 | ml |
Adjust pH to 2.5 and filter-sterilize. Prepare the solution just prior to use.
Aseptically combine solution A and solution B (freshly prepared). Then, add the following solutions (autoclaved):
Trace elements solution (see Medium No. 1182) | 1.0 | ml |
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