Medium data

Search for medium no. = [1197].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1197 ACIDIHALOBACTER PROSPERUS F5 MEDIUM

Solution A:

NaCl	`25.0`	g
(NH₄)₂SO₄	`0.4`	g
MgSO₄·7H₂O	`0.4`	g
K₂HPO₄	`0.4`	g
Distilled water	`900.0`	ml

Adjust pH 2.5 with H₂SO₄ and autoclave.
Solution B:

FeSO₄·7H₂O	`13.9`	g
K₂S₄O₆	`1.51`	g
Distilled water	`100.0`	ml

Adjust pH to 2.5 and filter-sterilize. Prepare the solution just prior to use.
Aseptically combine solution A and solution B (freshly prepared). Then, add the following solutions (autoclaved):

Trace elements solution (see Medium No. 1182) 1.0 ml