Medium data

Search for medium no. = [1182].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1182   FERROUS IRON YEAST EXTRACT MEDIUM

MgSO4·7H2O  0.5     g
(NH4)2SO4  0.45    g
Na2SO4  0.15    g
KH2PO4  0.05    g
KCl  0.05    g
Ca(NO3)2·4H2O  0.01    g
Trace elements solution (see below)  1.0     ml
Distilled water  1.0     L
Mix components thoroughly, adjust pH 2.0 with H2SO4 and autoclave. After cooling, aseptically add the following solutions (autoclave or *filer-sterilized):
1 M FeSO4 solution* (pH 2.0)  10.0     ml
1.0% Yeast extract solution  20.0     ml
Trace element solution:
ZnSO4·7H2O  10.0     g
CuSO4·5H2O  1.0     g
MnSO4·xH2O  1.0     g
CoSO4·7H2O  1.0     g
Cr2(SO4)3·xH2O  0.5     g
H3BO3  0.6     g
Na2MoO4·2H2O  0.5     g
NiSO4·6H2O  1.0     g
Na2SeO4  1.0     g
Na2WO4·2H2O  1.0     g
NaVO3  0.1     g
0.01 M H2SO4  1.0     L



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