Medium data
Search for medium no. = [1182].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1182 FERROUS IRON YEAST EXTRACT MEDIUM
| MgSO4·7H2O | 0.5 | g |
| (NH4)2SO4 | 0.45 | g |
| Na2SO4 | 0.15 | g |
| KH2PO4 | 0.05 | g |
| KCl | 0.05 | g |
| Ca(NO3)2·4H2O | 0.01 | g |
| Trace elements solution (see below) | 1.0 | ml |
| Distilled water | 1.0 | L |
Mix components thoroughly, adjust pH 2.0 with H2SO4 and autoclave. After cooling, aseptically add the following solutions (autoclave or *filer-sterilized):
| 1 M FeSO4 solution* (pH 2.0) | 10.0 | ml |
| 1.0% Yeast extract solution | 20.0 | ml |
Trace element solution:
| ZnSO4·7H2O | 10.0 | g |
| CuSO4·5H2O | 1.0 | g |
| MnSO4·xH2O | 1.0 | g |
| CoSO4·7H2O | 1.0 | g |
| Cr2(SO4)3·xH2O | 0.5 | g |
| H3BO3 | 0.6 | g |
| Na2MoO4·2H2O | 0.5 | g |
| NiSO4·6H2O | 1.0 | g |
| Na2SeO4 | 1.0 | g |
| Na2WO4·2H2O | 1.0 | g |
| NaVO3 | 0.1 | g |
| 0.01 M H2SO4 | 1.0 | L |
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