Medium data

Search for medium no. = [1190].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1190   VALLITALEA 70B-A MEDIUM

HEPES  4.7     g
NH4Cl  0.5     g
KH2PO4  0.3     g
NaCl  25.0     g
KCl  0.3     g
Yeast extract  0.2     g
Trace element solution (see Medium No. 439)  1.0     ml
Resazurin  1.0     mg
Distilled water  950.0     ml
Mix components thoroughly, adjust pH to 7.9 with NaOH and autoclave under a N2 atmosphere. After cooling, add the following solutions:
1 M Glucose solution  20.0     ml
5% MgCl2·6H2O solution  20.0     ml
1% CaCl2·2H2O solution  10.0     ml
Aseptically and anaerobically distribute the medium into culture vessels under a N2 gas stream and seal with butyl rubber stoppers. Prior inoculation, add per liter the following solution from an anaerobic stock (autoclaved and stored under a N2 atmosphere):
5% Na2S·9H2O solution  10.0     ml