Medium data

Search for medium no. = [1187].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1187   MARINE CLOSTRIDIUM MEDIUM

KH2PO4  0.1     g
MgCl2·6H2O  4.0     g
CaCl2·2H2O  1.0     g
NH4Cl  0.5     g
NaCl  25.0     g
Yeast extract  1.0     g
Peptone  1.0     g
Trace vitamins solution (see Medium No. 284)  2.0     ml
Trace mineral solution (see Medium No. 852)  1.0     ml
Se/W solution (Medium No. 852)  1.0     ml
Resazurin  1.0     mg
Distilled water  1.0     L
Mix components and adjust pH to 7.5. Bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels (e.g., 20 ml medium in 60 ml serum bottles) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized):
8% NaHCO3 solution*  25.0     ml
5% L-Cysteine·HCl·H2O solution  6.0     ml
5% Na2S·9H2O solution  6.0     ml