Medium data

Search for medium no. = [1183].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1183   CHLOROFLEXUS MEDIUM

KH2PO4  0.5      g
NH4Cl  0.5      g
MgCl2·6H2O  0.3      g
KCl  0.5      g
NaCl  0.5      g
Na2S2O3·5H2O  0.1      g
Yeast extract (BD-Difco)  1.0      g
HEPES  3.0      g
Trace vitamins solution (see below)  1.0      ml
Trace elements solution (see Medium No. 310)  1.0      ml
Distilled water  1.0      L
After autoclaving, add 1 ml of 5% Na2S·9H2O solution (autoclaved), 1 ml of 5% CaCl2·2H2O solution (autoclaved) and 10 ml of 3% NaHCO3 solution (filter-sterilized). Adjust pH to 7.5.
Trace vitamins solution:
Biotin  2.0      mg
Folic acid  2.0      mg
Pyridoxine·HCl  10.0      mg
Thiamine·HCl  5.0      mg
Riboflavin  5.0      mg
Nicotinic acid  5.0      mg
DL-Calcium pantothenate  5.0      mg
Vitamin B12  0.5      mg
p-Aminobenzoic acid  5.0      mg
Distilled water  100.0      ml