Medium data
Search for medium no. = [1183].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1183 CHLOROFLEXUS MEDIUM
| KH2PO4 | 0.5 | g |
| NH4Cl | 0.5 | g |
| MgCl2·6H2O | 0.3 | g |
| KCl | 0.5 | g |
| NaCl | 0.5 | g |
| Na2S2O3·5H2O | 0.1 | g |
| Yeast extract (BD-Difco) | 1.0 | g |
| HEPES | 3.0 | g |
| Trace vitamins solution (see below) | 1.0 | ml |
| Trace elements solution (see Medium No. 310) | 1.0 | ml |
| Distilled water | 1.0 | L |
After autoclaving, add 1 ml of 5% Na2S·9H2O solution (autoclaved), 1 ml of 5% CaCl2·2H2O solution (autoclaved) and 10 ml of 3% NaHCO3 solution (filter-sterilized). Adjust pH to 7.5.
Trace vitamins solution:
| Biotin | 2.0 | mg |
| Folic acid | 2.0 | mg |
| Pyridoxine·HCl | 10.0 | mg |
| Thiamine·HCl | 5.0 | mg |
| Riboflavin | 5.0 | mg |
| Nicotinic acid | 5.0 | mg |
| DL-Calcium pantothenate | 5.0 | mg |
| Vitamin B12 | 0.5 | mg |
| p-Aminobenzoic acid | 5.0 | mg |
| Distilled water | 100.0 | ml |
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