Medium data
Search for medium no. = [1172].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1172 MODIFIED MEDIUM FOR MARINE SPIROCHAETES
| NH4Cl | 0.5 | g |
| KH2PO4 | 0.1 | g |
| NaCl | 40.0 | g |
| MgCl2·6H2O | 4.0 | g |
| CaCl2·2H2O | 1.0 | g |
| Yeast extract | 1.0 | g |
| Maltose | 1.7 | g |
| Trace mineral solution (see Medium No. 852) | 1.0 | ml |
| Trace vitamins solution (see Medium No. 284) | 2.0 | ml |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Mix components and adjust pH to 7.5. Bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. Add per liter the following solutions (autoclaved or *filter-sterilized):
| 8% NaHCO3 solution* | 25.0 | ml |
| 5% L-Cysteine·HCl·H2O solution | 6.0 | ml |
| 5% Na2S·9H2O solution | 6.0 | ml |
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