Medium data

Search for medium no. = [1171].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1171 M39 METHYLOMONAS MEDIUM

MgSO₄·7H₂O	`0.099`	g
CaCl₂·2H₂O	`0.147`	g
K₂SO₄	`0.349`	g
NaNO₃	`0.17`	g
Distilled water	`1.0`	L

Mix components thoroughly. For preparation of solid medium, add 15.0 g/L agarose. Autoclave and, after cooling to 60C, add the following solutions (autoclaved or *filter-sterilized):

1.0 M KH₂PO₄ solution	`1.0`	ml
Selenite-Tungstate solution* (see Medium No. 431)	`1.0`	ml
Trace element solution* (see below)	`1.0`	ml
Vitamins solution* (see below)	`0.1`	ml
0.2 M CeCl₃ solution*	`0.1`	ml
1.0 M NaHCO₃ solution*	`3.0`	ml

Adjust pH to 6.5 - 7.0 with 0.2 N H₂SO₄ solution (autoclaved). Cultivate under an air containing 10% (v/v) methane in gas tight flasks or jars leaving ≥ 80% gas phase by volume.
Trace element solution:

EDTA·Na₂	`3.0`	g
FeCl₂·4H₂O	`1.5`	g
SL-6 trace element solution (see Medium No. 167)	`1.0`	L

Vitamins solution:

Biotin	`0.1`	mg
Folic acid	`0.1`	mg
Pyridoxine·HCl	`0.5`	mg
Thiamine·HCl	`0.25`	mg
Riboflavin	`0.25`	mg
Nicotinic acid	`0.25`	mg
Calcium pantothenate	`0.25`	mg
Vitamin B₁₂	`0.05`	mg
p-Aminobenzoic acid	`0.25`	mg
Lipoic acid	`0.25`	mg
1,4-Naphthoquinone	`0.2`	mg
Nicotinamide	`0.25`	mg
Hemin	`0.05`	mg
Distilled water	`1.0`	L