Medium data

Search for medium no. = [1167].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1167 MMFC1 MEDIUM

KH₂PO₄	`0.227`	g
K₂HPO₄	`0.348`	g
NH₄Cl	`0.5`	g
NaCl	`2.25`	g
MgSO₄·7H₂O	`0.5`	g
CaCl₂·2H₂O	`0.025`	g
Trace mineral solution (see below)	`1.0`	ml
Yeast extract	`2.0`	g
Resazurin	`0.5`	mg
Distilled water	`1.0`	L

Prepare medium under a N₂ gas atmosphere. After autoclaving, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized):

Trace vitamins* (see Medium No. 197)	`20.0`	ml
50% (v/v) Methanol solution	`8.0`	ml
5% L-Cysteine·HCl·H₂O solution	`0.35`	ml

Adjust final pH of the medium to 6.5, if necessary.
Trace mineral solution:

Na₂-EDTA	`22.59`	mg
MgSO₄·7H₂O	`246.0`	mg
CaCl₂·2H₂O	`14.0`	mg
H₃BO₃	`3.5`	mg
NaCl	`0.584`	mg
FeSO₄·7H₂O	`1.5`	mg
CoCl₂·6H₂O	`1.2`	mg
NiCl₂·6H₂O	`1.19`	mg
Na₂MoO₄·2H₂O	`0.944`	mg
Na₂SeO₃·5H₂O	`0.395`	mg
MnCl₂·4H₂O	`0.257`	mg
ZnCl₂	`0.136`	mg
CuCl₂·2H₂O	`0.205`	mg
Distilled water	`1.0`	L