Medium data

Search for medium no. = [1167].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1167   MMFC1 MEDIUM

KH2PO4  0.227   g
K2HPO4  0.348   g
NH4Cl  0.5     g
NaCl  2.25    g
MgSO4·7H2O  0.5     g
CaCl2·2H2O  0.025   g
Trace mineral solution (see below)  1.0     ml
Yeast extract  2.0     g
Resazurin  0.5     mg
Distilled water  1.0     L
Prepare medium under a N2 gas atmosphere. After autoclaving, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized):
Trace vitamins* (see Medium No. 197)  20.0     ml
50% (v/v) Methanol solution  8.0     ml
5% L-Cysteine·HCl·H2O solution  0.35    ml
Adjust final pH of the medium to 6.5, if necessary.
Trace mineral solution:
Na2-EDTA  22.59    mg
MgSO4·7H2O  246.0     mg
CaCl2·2H2O  14.0     mg
H3BO3  3.5     mg
NaCl  0.584   mg
FeSO4·7H2O  1.5     mg
CoCl2·6H2O  1.2     mg
NiCl2·6H2O  1.19    mg
Na2MoO4·2H2O  0.944   mg
Na2SeO3·5H2O  0.395   mg
MnCl2·4H2O  0.257   mg
ZnCl2  0.136   mg
CuCl2·2H2O  0.205   mg
Distilled water  1.0     L



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