Medium data

Search for medium no. = [1166].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1166   DESULFOVIBRIO DECHLORACETIVORANS MEDIUM

KH2PO4  0.25    g
K2HPO4  0.25    g
NH4Cl  0.5     g
NaCl  10.0     g
Na2SO4  2.8     g
MgCl2·6H2O  0.5     g
MgSO4·7H2O  0.2     g
CaCl2·2H2O  0.005   g
Trace metal solution SL12 (see Medium No. 576)  1.0     ml
Sodium lactate  1.12    g
Selenite-tungstate solution (see Medium No. 431)  0.4     ml
Resazurin  1.0     mg
Distilled water  1.0     L
Mix components thoroughly, distribute the medium into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized):
8% NaHCO3 solution*  30.0     ml
Vitamin solution* (see Medium No. 556)  1.0     ml
5% Na2S·9H2O solution  4.0     ml
Adjust pH to 7.1 - 7.2, if necessary.