Medium data

Search for medium no. = [576].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

576   DESULFATIBACILLUS OLEFINIVORANS MEDIUM

Solution A:
Na2SO4  3.0     g
KH2PO4  0.2     g
NH4Cl  0.3     g
NaCl  7.0     g
KCl  0.5     g
CaCl2·2H2O  0.15    g
Yeast extract (BD-Difco)  0.1     g
Trace element solution SL12 (see below)  1.0     ml
Selenite-tungstate solution (see Medium No. 431)  1.0     ml
HEPES  2.38    g
Resazurin  1.0     mg
Distilled water  960.0     ml
Mix ingredients, adjust pH to 7.5 and autoclave under a N2 atmosphere. After cooling, add the following stock solutions (filter-sterilized or *autoclaved) to the solution:
15% MgCl2·6H2O solution*  10.0     ml
8% NaHCO3 solution  30.0     ml
Vitamin solution (see Medium No. 403)  1.0     ml
Thiamine solution (see Medium No. 403)  1.0     ml
Vitamin B12 solution (see Medium No. 403)  1.0     ml
Substrate:
Sodium palmitate  2.8     g
Autoclave in culture vessels.
To complete the medium, combine Solution A and substrate in the culture vessels under a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Finally, reduce the medium with 0.01 volume of 5% Na2S·9H2O solution (autoclaved and stored under a N2 atmosphere).
Trace metal solution SL12:
EDTA·2Na  3.0     g
FeSO4·7H2O  1.1     g
CoCl2·6H2O  190.0     mg
MnCl2·4H2O  50.0     mg
ZnCl2  42.0     mg
NiCl2·6H2O  24.0     mg
Na2MoO4·2H2O  18.0     mg
H3BO3  300.0     mg
CuCl2·2H2O  2.0     mg
Distilled water  1.0     L