Medium data

Search for medium no. = [576].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


Solution A:
Na2SO4  3.0     g
KH2PO4  0.2     g
NH4Cl  0.3     g
NaCl  7.0     g
KCl  0.5     g
CaCl2·2H2O  0.15    g
Yeast extract (BD-Difco)  0.1     g
Trace element solution SL12 (see below)  1.0     ml
Selenite-tungstate solution (see Medium No. 431)  1.0     ml
HEPES  2.38    g
Resazurin  1.0     mg
Distilled water  960.0     ml
Mix ingredients, adjust pH to 7.5 and autoclave under a N2 atmosphere. After cooling, add the following stock solutions (filter-sterilized or *autoclaved) to the solution:
15% MgCl2·6H2O solution*  10.0     ml
8% NaHCO3 solution  30.0     ml
Vitamin solution (see Medium No. 403)  1.0     ml
Thiamine solution (see Medium No. 403)  1.0     ml
Vitamin B12 solution (see Medium No. 403)  1.0     ml
Sodium palmitate  2.8     g
Autoclave in culture vessels.
To complete the medium, combine Solution A and substrate in the culture vessels under a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Finally, reduce the medium with 0.01 volume of 5% Na2S·9H2O solution (autoclaved and stored under a N2 atmosphere).
Trace metal solution SL12:
EDTA·2Na  3.0     g
FeSO4·7H2O  1.1     g
CoCl2·6H2O  190.0     mg
MnCl2·4H2O  50.0     mg
ZnCl2  42.0     mg
NiCl2·6H2O  24.0     mg
Na2MoO4·2H2O  18.0     mg
H3BO3  300.0     mg
CuCl2·2H2O  2.0     mg
Distilled water  1.0     L