Medium data
Search for medium no. = [576].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
576 DESULFATIBACILLUS OLEFINIVORANS MEDIUM
Solution A:
| Na2SO4 | 3.0 | g |
| KH2PO4 | 0.2 | g |
| NH4Cl | 0.3 | g |
| NaCl | 7.0 | g |
| KCl | 0.5 | g |
| CaCl2·2H2O | 0.15 | g |
| Yeast extract (BD-Difco) | 0.1 | g |
| Trace element solution SL12 (see below) | 1.0 | ml |
| Selenite-tungstate solution (see Medium No. 431) | 1.0 | ml |
| HEPES | 2.38 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 960.0 | ml |
Mix ingredients, adjust pH to 7.5 and autoclave under a N2 atmosphere. After cooling, add the following stock solutions (filter-sterilized or *autoclaved) to the solution:
| 15% MgCl2·6H2O solution* | 10.0 | ml |
| 8% NaHCO3 solution | 30.0 | ml |
| Vitamin solution (see Medium No. 403) | 1.0 | ml |
| Thiamine solution (see Medium No. 403) | 1.0 | ml |
| Vitamin B12 solution (see Medium No. 403) | 1.0 | ml |
Substrate:
Autoclave in culture vessels.
To complete the medium, combine Solution A and substrate in the culture vessels under a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Finally, reduce the medium with 0.01 volume of 5% Na2S·9H2O solution (autoclaved and stored under a N2 atmosphere).
Trace metal solution SL12:
| EDTA·2Na | 3.0 | g |
| FeSO4·7H2O | 1.1 | g |
| CoCl2·6H2O | 190.0 | mg |
| MnCl2·4H2O | 50.0 | mg |
| ZnCl2 | 42.0 | mg |
| NiCl2·6H2O | 24.0 | mg |
| Na2MoO4·2H2O | 18.0 | mg |
| H3BO3 | 300.0 | mg |
| CuCl2·2H2O | 2.0 | mg |
| Distilled water | 1.0 | L |
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