Medium data

Search for medium no. = [1150].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1150   MEDIUM FOR MARINE SPIROCHAETES

NH4Cl  0.5     g
KH2PO4  0.1     g
NaCl  40.0     g
MgCl2·6H2O  4.0     g
CaCl2·2H2O  1.0     g
Yeast extract  1.0     g
Glucose  1.7     g
Trace mineral solution (see Medium No. 852)  1.0     ml
Se/W solution (see Medium No. 852)  1.0     ml
Trace vitamins solution (see Medium No. 284)  1.0     ml
Resazurin  1.0     mg
Distilled water  1.0     L
Mix components and adjust pH to 7.5. Bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. Add per liter the following solutions (autoclaved or *filter-sterilized):
8% NaHCO3 solution*  25.0     ml
5% L-Cysteine·HCl·H2O solution  6.0     ml
5% Na2S·9H2O solution  6.0     ml