Medium data

Search for medium no. = [1139].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1139 NATRONOSPIRA PROTEINIVORA MEDIUM

Basic mineral salt medium 1 (see below)	`500.0`	ml
Basal medium 2 (see below)	`500.0`	ml
10% Tryptone solution (autoclaved)	`15.0`	ml
1.0 Yeast extract solution (autoclaved)	`2.0`	ml

Aseptically combine the above described media and solutions.
Basic mineral salt medium 1:

NaCl	`240.0`	g
K₂HPO₄	`2.5`	g
(NH₄)₂SO₄	`0.5`	g

Add components to distilled water and bring volume to 1.0 L. Adjust pH to 6.8 - 6.9 by 1 M K₂HPO₄ and autoclave. After cooling, add the following solutions (autoclaved):

1 M MgCl₂ solution	`1.0`	ml
Trace element solution (see Medium No. 1079)	`1.0`	ml

Basal medium 2:

Na₂CO₃	`190.0`	g
NaHCO₃	`30.0`	g
NaCl	`16.0`	g
K₂HPO₄	`2.0`	g

Add components to distilled water and bring volume to 1.0 L. Check pH of the medium to be 9.9 - 10.0. Autoclave and let it stand for three days. Decant the clear solution into another empty sterile bottle. Aseptically add per liter the following solutions (autoclaved):

2 M NH₄Cl solution	`4.0`	ml
1 M MgCl₂ solution	`1.0`	ml
Trace element solution (see Medium No. 1079)	`2.0`	ml

Casein solution:

Casein	`5.0`	g
Distilled water	`100.0`	ml

Suspend casein in distilled water and keep at 50C. Titrate pH to 8.2 - 8.5 with 1 M NaOH solution, until pH is stabilized. Autoclave the solution at 120C for 40 min in a closed bottle.