Medium data

Search for medium no. = [1113].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1113 ANAEROBIC MINERAL MEDIUM WITH YEAST EXTRACT AND PEPTONE

NaCl	`2.5`	g
KCl	`0.3`	g
K₂HPO₄	`0.3`	g
MgSO₄·7H₂O	`0.1`	g
NH₄Cl	`1.0`	g
CaSO₄·2H₂O	`12.5`	mg
Yeast extract	`0.1`	g
Fatty acid mixture (see Medium No. 266)	`10.0`	ml
Trace vitamins (see Medium No. 197)	`2.0`	ml
Trace minerals solution (see Medium No. 228)	`1.0`	ml
Resazurin	`0.5`	mg
Distilled water	`1.0`	L

Mix components, adjust pH to 7.0 and autoclave under a N₂ atmosphere in a bottle capped with a butyl rubber stopper. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):

Trace vitamins* (see Medium No. 197)	`2.0`	ml
10% Peptone solution	`30.0`	ml
1.0 M Sodium thiosulfate solution	`10.0`	ml
5% Na₂S·9H₂O solution (pH 7.0)	`8.0`	ml