Medium data

Search for medium no. = [1112].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1112   MINERAL MEDIUM WITH GLUCOSE

NaCl  2.5     g
KCl  0.3     g
K2HPO4  0.3     g
MgSO4·7H2O  0.1     g
NH4Cl  1.0     g
CaSO4·7H2O  12.5     mg
Yeast extract  0.1     g
Trace vitamins (see Medium No. 197)  2.0     ml
Trace minerals solution (see Medium No. 228)  1.0     ml
Distilled water  1.0     L
Mix components thoroughly, adjust pH to 7.4 and autoclave. After cooling, add the following solution (autoclaved):
1 M Glucose solution  20.0     ml