Medium data

Search for medium no. = [1104].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1104   HYPNOCYCLICUS MEDIUM

Na2SO4  0.02    g
KH2PO4  1.0     g
NH4Cl  0.1     g
NaCl  20.0     g
MgCl2·6H2O  3.0     g
KCl  0.5     g
CaCl2·2H2O  0.15    g
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Resazurin  1.0     mg
Distilled water  950.0     ml
Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) atmosphere. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
8% NaHCO3 solution*  30.0     ml
Vitamin solution* (see Medium No. 556)  1.0     ml
10% Yeast extract solution  5.0     ml
1 M Maltose solution  5.0     ml
5% Na2S·9H2O solution  10.0     ml
Readjust pH to 6.8 - 7.2, if necessary.



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