Medium data
Search for medium no. = [1104].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1104 HYPNOCYCLICUS MEDIUM
Na2SO4 | 0.02 | g |
KH2PO4 | 1.0 | g |
NH4Cl | 0.1 | g |
NaCl | 20.0 | g |
MgCl2·6H2O | 3.0 | g |
KCl | 0.5 | g |
CaCl2·2H2O | 0.15 | g |
FeCl2 solution (see Medium No. 187) | 1.0 | ml |
Trace element solution (see Medium No. 187) | 1.0 | ml |
Resazurin | 1.0 | mg |
Distilled water | 950.0 | ml |
Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) atmosphere. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
8% NaHCO3 solution* | 30.0 | ml |
Vitamin solution* (see Medium No. 556) | 1.0 | ml |
10% Yeast extract solution | 5.0 | ml |
1 M Maltose solution | 5.0 | ml |
5% Na2S·9H2O solution | 10.0 | ml |
Readjust pH to 6.8 - 7.2, if necessary.
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