Medium data

Search for medium no. = [1102].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1102   ANAEROBIC NATURAL SEAWATER MEDIUM

Tryptone  5.0     g
Yeast extract  1.0     g
Resazurin  0.5     mg
Natural seawater (filtrated)  750.0     ml
Distilled water  250.0     ml
Mix components thoroughly and adjust pH to 7.0. Bring to a boil and cool down under a N2 gas atmosphere. Distribute the medium in culture vessels (e.g., 10 ml in Balch tubes) under N2, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved and stored under a N2 atmosphere):
5% L-Cysteine·HCl·H2O solution  6.0     ml
5% Na2S·9H2O solution  6.0     ml
Comment: For strain JCM 32399, supplement with 10 mM sodium pyruvate. For JCM 33129, omit resazurin and the reducing agents (L-cysteine·HCl·H2O and Na2S·9H2O). For JCM 33131, supplement with 10 ml/L trace vitamins (see Medium No. 197).



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