Medium data

Search for medium no. = [1085].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1085   ANAEROBACTER MEDIUM

NH4Cl  0.33    g
KH2PO4  0.33    g
KCl  0.33    g
CaCl2·2H2O  0.33    g
MgCl2·6H2O  0.33    g
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Yeast extract  1.0     g
Resazurin  1.0     mg
Distilled water  1.0     L
Mix components thoroughly, adjust pH to 7.0 and autoclave under a N2 atmosphere. After cooling, aseptically and anaerobically add per liter the following sterile solutions from anaerobic stocks (autoclaved and stored under a N2 atmosphere or *filter-sterilized and stored under 80% N2 and 20% CO2 gas mixture):
20% (w/v) sucrose solution  10.0     ml
8% NaHCO3 solution*  18.3     ml
Dispense the medium into culture vessels under a N2 atmosphere and seal with butyl rubber stoppers. Finally, add per litter the following solutions (autoclaved and stored under a N2 atmosphere):
5% L-Cysteine·HCl·H2O solution  6.0     ml
5% Na2S·9H2O solution  6.0     ml