Medium data
Search for medium no. = [1085].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1085 ANAEROBACTER MEDIUM
| NH4Cl | 0.33 | g |
| KH2PO4 | 0.33 | g |
| KCl | 0.33 | g |
| CaCl2·2H2O | 0.33 | g |
| MgCl2·6H2O | 0.33 | g |
| FeCl2 solution (see Medium No. 187) | 1.0 | ml |
| Trace element solution (see Medium No. 187) | 1.0 | ml |
| Yeast extract | 1.0 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Mix components thoroughly, adjust pH to 7.0 and autoclave under a N2 atmosphere. After cooling, aseptically and anaerobically add per liter the following sterile solutions from anaerobic stocks (autoclaved and stored under a N2 atmosphere or *filter-sterilized and stored under 80% N2 and 20% CO2 gas mixture):
| 20% (w/v) sucrose solution | 10.0 | ml |
| 8% NaHCO3 solution* | 18.3 | ml |
Dispense the medium into culture vessels under a N2 atmosphere and seal with butyl rubber stoppers. Finally, add per litter the following solutions (autoclaved and stored under a N2 atmosphere):
| 5% L-Cysteine·HCl·H2O solution | 6.0 | ml |
| 5% Na2S·9H2O solution | 6.0 | ml |
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