Medium data

Search for medium no. = [1083].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1083 DESULFURELLA TR1 MEDIUM

Na₂HPO₄·2H₂O	`0.53`	g
KH₂PO₄	`0.41`	g
NH₄Cl	`0.3`	g
NaCl	`0.3`	g
MgCl₂·6H₂O	`0.1`	g
CaCl₂·2H₂O	`0.11`	g
Acid trace element solution (see Medium No. 602)	`1.0`	ml
Alkaline trace element solution (see Medium No. 602)	`1.0`	ml
Sodium acetate·3H₂O	`0.68`	g
Yeast extract	`0.1`	g
Sulfur (powder)	`0.8`	g
Resazurin	`1.0`	m
Distilled water	`1.0`	L

Mix components, except sulfur, and autoclave under a N₂-CO₂ (4:1, v/v) gas mixture. After cooling, add the following solutions (filter-sterilized):

Vitamin solution (see Medium No. 602)	`0.2`	ml
8% NaHCO₃ solution	`12.5`	ml

Steam sulfur for 3 hr on each of 3 successive days. Aseptically and anaerobically distribute the medium and sulfur into culture vessels (e.g., 50 ml of the medium and 0.04 g of sulfur in 120 ml serum bottle) under a N₂-CO₂ (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Prior to use, anaerobically add per liter the following solution from an anaerobic stock (autoclaved and stored under a N₂ atmosphere):

5% Na₂S·9H₂O solution 6.0 ml

Adjust pH to 6.0, if necessary.