Medium data

Search for medium no. = [1083].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1083   DESULFURELLA TR1 MEDIUM

Na2HPO4·2H2O  0.53    g
KH2PO4  0.41    g
NH4Cl  0.3     g
NaCl  0.3     g
MgCl2·6H2O  0.1     g
CaCl2·2H2O  0.11    g
Acid trace element solution (see Medium No. 602)  1.0     ml
Alkaline trace element solution (see Medium No. 602)  1.0     ml
Sodium acetate·3H2O  0.68    g
Yeast extract  0.1     g
Sulfur (powder)  0.8     g
Resazurin  1.0     m
Distilled water  1.0     L
Mix components, except sulfur, and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions (filter-sterilized):
Vitamin solution (see Medium No. 602)  0.2     ml
8% NaHCO3 solution  12.5     ml
Steam sulfur for 3 hr on each of 3 successive days. Aseptically and anaerobically distribute the medium and sulfur into culture vessels (e.g., 50 ml of the medium and 0.04 g of sulfur in 120 ml serum bottle) under a N2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Prior to use, anaerobically add per liter the following solution from an anaerobic stock (autoclaved and stored under a N2 atmosphere):
5% Na2S·9H2O solution  6.0     ml
Adjust pH to 6.0, if necessary.