Medium data

Search for medium no. = [602].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

602 DESULFOVIBRIO MAGNUS MEDIUM

Solution A:

Na₂HPO₄·2H₂O	`0.53`	g
KH₂PO₄	`0.41`	g
NH₄Cl	`0.3`	g
NaCl	`0.3`	g
Na₂SO₄	`1.42`	g
Sodium lactate	`2.24`	g
Acid trace element solution (see below)	`1.0`	ml
Alkaline trace element solution (see below)	`1.0`	ml
Distilled water	`900.0`	ml

Mix components and autoclave under a N₂ gas atmosphere.
Acid trace element solution:

6 N HCl	`10.0`	ml
H₃BO₃	`61.8`	mg
MnCl₂·4H₂O	`61.25`	mg
FeCl₂·4H₂O	`943.5`	mg
CoCl₂·6H₂O	`64.5`	mg
NiCl₂·6H₂O	`12.86`	mg
ZnCl₂	`67.7`	mg
Distilled water	`1.0`	L

Alkaline trace element solution:

NaOH	`400.0`	mg
Na₂SeO₃·5H₂O	`17.3`	mg
Na₂WO₄·2H₂O	`29.4`	mg
Na₂MoO₄·2H₂O	`20.5`	mg
distilled water	`1.0`	L

Solution B:

CaCl₂·2H₂O	`0.11`	g
MgCl₂·6H₂O	`0.1`	g
Distilled water	`50.0`	ml

Mix components and autoclave under a N₂ atmosphere.
Solution C:

NaHCO₃	`2.0`	g
Distilled water	`50.0`	ml

Mix components, filter-sterilize and store under a N₂-CO₂ (4:1, v/v) gas mixture.
To complete the medium, combine Solutions A, B, C and 1 ml Vitamin solution (see below) aseptically under a stream of a N₂-CO₂ (4:1, v/v) gas mixture. Distribute the medium into sterilized culture vessels under the same gas mixture and seal with butyl rubber stoppers. Then, add 0.01 volume of 5% Na₂S·9H₂O solution (autoclaved under a N₂ atmosphere). Check the final pH to be 7.0 - 7.2. Pressurize the inoculated vessels to 70 kPa N₂-CO₂ (4:1, v/v) overpressure.
Vitamin solution:

Biotin	`20.0`	mg
Nicotinic acid	`200.0`	mg
p-Aminobenzoic acid	`100.0`	mg
Thiamine·HCl	`200.0`	mg
DL-Calcium pantothenate	`100.0`	mg
Pyridoxine·HCl	`500.0`	mg
Vitamin B₁₂	`100.0`	mg
Riboflavin	`100.0`	mg
Distilled water	`1.0`	L

Filter-sterilize and store under a N₂ atmosphere.