Medium data

Search for medium no. = [1078].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1078   ALKALIPHILUS 3B MEDIUM

NaCl  1.0     g
NH4Cl  1.0     g
KH2PO4  0.1     g
K2HPO4  0.1     g
KCl  0.1     g
MgCl2·6H2O  0.1     g
Tris base  3.0     g
Na2S2O3·5H2O  1.5     g
Trace element solution (see Medium No. 439)  1.0     ml
Resazurin  1.0     mg
Distilled water  910.0     ml
Mix components thoroughly, adjust pH to 9.0 with NaOH and autoclave under a N2 atmosphere. After cooling, replace the gas phase with a N2-CO2 (4:1, v/v) gas mixture. Add the following solutions from sterile anaerobic stocks (autoclaved and stored under a N2 atmosphere):
10% (w/v) Yeast extract solution  50.0     ml
0.5 M Crotonic acid (pH 7.0)  20.0     ml
8% Na2CO3 solution  20.0     ml
5% Na2S·9H2O solution  6.0     ml
Check final pH be around 8.5.