Medium data
Search for medium no. = [1074].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1074 VULCANIBACILLUS B5 MEDIUM
| NH4Cl | 0.5 | g |
| KCl | 0.5 | g |
| KH2PO4 | 0.2 | g |
| CaCl2·2H2O | 0.1 | g |
| MgCl2·6H2O | 0.4 | g |
| NaCl | 1.0 | g |
| NaNO3 | 3.0 | g |
| Yeast extract | 1.0 | g |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| Trace element solution (see Medium No. 231) | 10.0 | ml |
| NaHCO3 | 2.5 | g |
| Na2S·9H2O | 0.3 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Mix ingredients except NaHCO3, Trace vitamins and Na2S·9H2O, and autoclave under a N2-CO2 (90:10) atmosphere. Filter-sterilize NaHCO3 as an 8.3% solution and Trace vitamins while gassing with N2. Separately autoclave Na2S·9H2O as a 3% solution under a N2 atmosphere. Aseptically and anaerobically add NaHCO3 and Trace vitamins to the medium under a N2-CO2 (90:10) atmosphere. Prior to inoculation, add the sterile Na2S·9H2O solution and adjust pH to 7.0.
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