Medium data

Search for medium no. = [1069].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1069   MICROAEROPHILIC LUTIBACTER MEDIUM

Artificial saltwater (see Medium No. 629)  1.0     L
MES  1.95    g
Wolfe's mineral solution (see Medium No. 265)  1.0     ml
Trace vitamins (see Medium No. 197)  10.0     ml
Tryptone (BD-Difco)  2.0     g
Mix components and adjust pH to 6.2 to 6.5. Distribute the medium into culture vessels, replace the gas phase with N2-CO2-O2 (90:10:2, v/v/v), seal with butyl rubber stoppers and autoclave.



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