Medium data
Search for medium no. = [1069].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1069 MICROAEROPHILIC LUTIBACTER MEDIUM
Artificial saltwater (see Medium No. 629) | 1.0 | L |
MES | 1.95 | g |
Wolfe's mineral solution (see Medium No. 265) | 1.0 | ml |
Trace vitamins (see Medium No. 197) | 10.0 | ml |
Tryptone (BD-Difco) | 2.0 | g |
Mix components and adjust pH to 6.2 to 6.5. Distribute the medium into culture vessels, replace the gas phase with N2-CO2-O2 (90:10:2, v/v/v), seal with butyl rubber stoppers and autoclave.
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