Medium data

Search for medium no. = [1068].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1068   SALTWATER IRON-OXIDIZING BACTERIA MEDIUM-II

Artificial saltwater (see Medium No. 629)  1.0     L
Wolfe's mineral solution (see Medium No. 265)  10.0     ml
Mix components, distribute the medium into culture vessels (e.g., 10 ml in Balch-type tubes), seal with butyl rubber stoppers and autoclave. After autoclaving, aseptically add per liter the following solutions (filter-sterilized):
Trace vitamins (see Medium No. 197)  10.0     ml
8% NaHCO3 solution  12.5     ml
0.2 M FeCl2 solution (freshly prepared)  10.0     ml
Replace the gas phase with a N2-CO2-O2 (80:20:3, v/v/v) gas mixture and pressurize to 200 kPa with the same gas mixture.