Medium data

Search for medium no. = [1068].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1068 SALTWATER IRON-OXIDIZING BACTERIA MEDIUM-II

Artificial saltwater (see Medium No. 629)	`1.0`	L
Wolfe's mineral solution (see Medium No. 265)	`10.0`	ml

Mix components, distribute the medium into culture vessels (e.g., 10 ml in Balch-type tubes), seal with butyl rubber stoppers and autoclave. After autoclaving, aseptically add per liter the following solutions (filter-sterilized):

Trace vitamins (see Medium No. 197)	`10.0`	ml
8% NaHCO₃ solution	`12.5`	ml
0.2 M FeCl₂ solution (freshly prepared)	`10.0`	ml

Replace the gas phase with a N₂-CO₂-O₂ (80:20:3, v/v/v) gas mixture and pressurize to 200 kPa with the same gas mixture.