Medium data

Search for medium no. = [1064].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


KCl  0.5     g
KH2PO4  0.2     g
NaCl  1.0     g
NH4Cl  0.05    g
CaCl2·2H2O  0.1     g
MgCl2·6H2O  0.4     g
Yeast extract  1.0     g
Glucose  0.36    g
Trace vitamins (see Medium No. 197)  1.0     ml
Trace element solution (see Medium No. 231)  1.0     ml
NaHCO3  0.84    g
Na2S·9H2O  0.5     g
Resazurin  1.0     mg
Distilled water  1.0     L
Mix ingredients except NaHCO3, Trace vitamins and Na2S·9H2O, and autoclave under a N2-CO2 (90:10) atmosphere. Filter-sterilize NaHCO3 as an 8.4% solution and Trace vitamins while gassing with N2. Separately autoclave Na2S·9H2O as 5% solution under a N2 atmosphere. Aseptically and anaerobically add NaHCO3 and Trace vitamins to the medium under a N2-CO2 (90:10) atmosphere. Prior to inoculation, add the sterile Na2S·9H2O solution, and adjust pH to 7.5 - 8.0.

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