Medium data

Search for medium no. = [1064].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1064 GEOSPOROBACTER MEDIUM

KCl	`0.5`	g
KH₂PO₄	`0.2`	g
NaCl	`1.0`	g
NH₄Cl	`0.05`	g
CaCl₂·2H₂O	`0.1`	g
MgCl₂·6H₂O	`0.4`	g
Yeast extract	`1.0`	g
Glucose	`0.36`	g
Trace vitamins (see Medium No. 197)	`1.0`	ml
Trace element solution (see Medium No. 231)	`1.0`	ml
NaHCO₃	`0.84`	g
Na₂S·9H₂O	`0.5`	g
Resazurin	`1.0`	mg
Distilled water	`1.0`	L

Mix ingredients except NaHCO₃, Trace vitamins and Na₂S·9H₂O, and autoclave under a N₂-CO₂ (90:10) atmosphere. Filter-sterilize NaHCO₃ as an 8.4% solution and Trace vitamins while gassing with N₂. Separately autoclave Na₂S·9H₂O as 5% solution under a N₂ atmosphere. Aseptically and anaerobically add NaHCO₃ and Trace vitamins to the medium under a N₂-CO₂ (90:10) atmosphere. Prior to inoculation, add the sterile Na₂S·9H₂O solution, and adjust pH to 7.5 - 8.0.